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Vinculin antibody
VCL
Reactivity: Human, Mouse, Rat
WB, IHC, ELISA, IF, FACS
Host: Mouse
Monoclonal
5E7
unconjugated
Product Details anti-Vinculin Antibody
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Target
See all Vinculin (VCL) Antibodies
Vinculin (VCL)
Reactivity
All reactivities for Vinculin antibodies
Human, Mouse, Rat
Host
All hosts for Vinculin antibodies
Mouse
Clonality
All clonalities for Vinculin antibodies
Monoclonal
Conjugate
All conjugates for Vinculin antibodies
This Vinculin antibody is un-conjugated
Application
All applications for Vinculin antibodies
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
Purification
Protein A+G purification
Purity
≥95 % as determined by SDS-PAGE
Immunogen
vinculin
Clone
5E7
Isotype
IgG1
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Alternatives
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Application Details
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Application Notes
WB: 1:2000-1:20000, IHC: 1:50-1:500, IF: 1:50-1:500
Restrictions
For Research Use only
Handling
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Format
Liquid
Buffer
PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
-20°C for 12 months (Avoid repeated freeze / thaw cycles.)
Expiry Date
12 months
Target Details for Vinculin
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Target
Vinculin (VCL)
Alternative Name
Vinculin (VCL Products )
Synonyms
CMD1W antibody, CMH15 antibody, MV antibody, MVCL antibody, VCL antibody, im:6902790 antibody, hm:zehn2160 antibody, DDBDRAFT_0186762 antibody, DDBDRAFT_0232320 antibody, DDB_0186762 antibody, DDB_0232320 antibody, 9430097D22 antibody, AA571387 antibody, AI462105 antibody, AW545629 antibody, CG3299 antibody, CT11081 antibody, Dmel\\CG3299 antibody, Dmvincp antibody, EG:103B4.1 antibody, Vin antibody, Vin2EF antibody, vinc antibody, LOC100228062 antibody, vcl-A antibody, vinculin antibody, vinculin a antibody, vinculin A antibody, Vinculin antibody, vinculin S homeolog antibody, VCL antibody, vcla antibody, vinA antibody, Vcl antibody, Vinc antibody, CpipJ_CPIJ013873 antibody, Bm1_37935 antibody, vcl antibody, vcl.S antibody
Background
Synonyms:CMD1W, Metavinculin, MVCL, VCL, Vinculin Background:Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament(F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117- kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin(124 kDa), is muscle-specific.
Molecular Weight
124 kDa
UniProt
P18206
Pathways
Cell-Cell Junction Organization , Maintenance of Protein Location , Signaling Events mediated by VEGFR1 and VEGFR2
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