SARS-CoV-2 Spike antibody

Catalog No. ABIN7121593

Product Details anti-SARS-CoV-2 Spike Antibody

Target: SARS-CoV-2 Spike

Reactivity: SARS-CoV-2

Host: Mouse

Clonality: Monoclonal

Conjugate: This SARS-CoV-2 Spike antibody is un-conjugated

Application: ELISA

Purpose: SARS-CoV-2 Whole Spike Protein Antibody

Specificity: Anti-SARS-CoV-2 Whole Spike antibody is directed against SARS Coronavirus 2 Spike (S) protein.

Cross-Reactivity (Details): No reactivity was seen against SARS COV-2 Spike S1 subunit, SARS COV-2 Spike S1 RBD, SARS COV-2 loop, SARS COV-2 (N), MERS CoV. Cross reactivity with homologues from other sources has not been determined.

Purification: The product was purified from roller bottle culture by protein A chromatography. BLAST analysis was used to suggest reactivity with related Coronavirus proteins.

Sterility: Sterile filtered

Immunogen: This protein A purified antibody was produced by repeated immunizations with a purified SARS-CoV-2 S protein with His-tag, derived from the transfected human HEK293 cells.

Clone: 15D9-E2-C10

Isotype: IgG

Application Details

Application Notes: Anti-SARS-CoV-2 Whole Spike protein antibody has been tested for use in ELISA and shows reactivity to SARS COV-2 Whole Spike and COV-2 Spike (S2) protein. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for SARS-CoV-2 Spike

Target: SARS-CoV-2 Spike

Abstract: SARS-CoV-2 Spike Products

Synonyms: E2 antibody, E2 glycoprotein precursor antibody, Surface Glycoprotein antibody, S antibody

Target Type: Viral Protein

Background: SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2) the virus that causes the disease COVID-19, is related to SARS-CoV, MERS-CoV, and other betacoronaviruses (HKU1, NL63, OC43 and 229E). SARS-CoV-2 is an enveloped positive strand RNA virus that consists of several structural proteins including spike (S) protein, envelope (E) protein, membrane (M) protein and nucleocapsid (N) protein; and other non-structural proteins (NSPs). Spike proteins located on the surface of the virus, have a high affinity for binding to human receptor ACE2 (angiotensin-converting enzyme 2) within respiratory epithelial which serves as an entry mechanism for infection. As such, the S protein may be vital to blocking viral infection and vaccine development.