CEACAM5 antibody (Alexa Fluor 488)
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- Target See all CEACAM5 Antibodies
- CEACAM5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CEACAM5 antibody is conjugated to Alexa Fluor 488
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), Test (T)
- Specificity
- This antibody recognizes proteins of 80-200 kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This MAb does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70 % are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix.
- Cross-Reactivity (Details)
- Human.
- Purification
- Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules.
- Immunogen
- Recombinant human CEA protein
- Isotype
- IgG2a
- Top Product
- Discover our top product CEACAM5 Primary Antibody
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- Application Notes
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Positive Control: MCF7 or 293T cells. Human colon carcinoma.
Known Application: Flow Cytometry (5 µL per test per one million cells or 5 µL per 100 µL of whole blood),Immunofluorescence (1:50-1:100),Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 100 μg/mL
- Buffer
- Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.
- Expiry Date
- 24 months
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- Target
- CEACAM5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5))
- Alternative Name
- CEACAM5 (CEACAM5 Products)
- Synonyms
- CD66e antibody, CEA antibody, CEACAM5 antibody, CEACAM antibody, 1600029H12Rik antibody, Psg30 antibody, carcinoembryonic antigen related cell adhesion molecule 5 antibody, carcinoembryonic antigen antibody, carcinoembryonic antigen-related cell adhesion molecule 5 antibody, carcinoembryonic antigen-related cell adhesion molecule 8 antibody, carcinoembryonic antigen gene family antibody, CEACAM5 antibody, Ceacam5 antibody, LOC100409241 antibody, Cea antibody
- Background
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Carcinoembryonic Antigen-related Cell Adhesion Molecule 5, CEACAM5, CD66, Biliary Glycoprotein (BGP-1),Carcinoembryonic Antigen (CEA) / CD66
Cellular localisation: Cytoplasmic and luminal surface - Molecular Weight
- 80-200kDa
- Gene ID
- 1048, 709196
- UniProt
- P06731
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