E2F6 antibody
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- Target See all E2F6 Antibodies
- E2F6 (E2F Transcription Factor 6 (E2F6))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This E2F6 antibody is un-conjugated
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Application
- Immunofluorescence (IF), Flow Cytometry (FACS)
- Specificity
- The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1and S phases of the cell cycle. E2F-1 is a member of a broader family of transcriptional regulators including E2F-2, E2F-3, E2F-4, E2F-5 and E2F-6, each of which forms heterodimers with a second protein, DP-1, forming an active E2F transcriptional regulatory complex.
- Cross-Reactivity (Details)
- Human
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant full-length human E2F6 protein
- Isotype
- IgG2b
- Top Product
- Discover our top product E2F6 Primary Antibody
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- Application Notes
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.
Positive_Control: HeLa or Jurkat cells.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- E2F6 (E2F Transcription Factor 6 (E2F6))
- Alternative Name
- E2F6 (E2F6 Products)
- Synonyms
- E2F-6 antibody, AI462434 antibody, E2F6b antibody, EMA antibody, E2F transcription factor 6 antibody, E2F6 antibody, E2f6 antibody
- Background
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EMA, MGC111545,E2F6
Cellular localisation: Nucleus - Molecular Weight
- 35kDa
- Gene ID
- 1876, 603093
- UniProt
- O75461
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