NR5A1 antibody (AA 220-461)

Catalog No. ABIN7126449

Product Details anti-NR5A1 Antibody

Target: NR5A1 (Nuclear Receptor Subfamily 5, Group A, Member 1 (NR5A1))

Binding Specificity: AA 220-461

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NR5A1 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: The protein encoded by this gene is a transcriptional activator involved in sex determination. The encoded protein binds DNA as a monomer. Defects in this gene are a cause of XY sex reversal with or without adrenal failure as well as adrenocortical insufficiency without ovarian defect. Steroidogenic Factor 1 (SF-1) is considered an orphan nuclear receptor that belongs to subfamily 5. It was found to be a regulator of steroidogenic enzyme gene expression. Oxysterols are suggested as its ligands. It is expressed in all steroidogenic tissues, including the adrenal cortex, testicular Sertoli cells, and Leydig cells, ovarian theca, hypothalamus, and anterior pituitary. SF-1 plays an important role in adrenal and gonadal development. SF-1 is highly valuable marker to determine the adrenocortical origin of an adrenal mass.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa220-461) of human SF-1 protein

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human testis or adrenal cortical carcinoma.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for NR5A1

Target: NR5A1 (Nuclear Receptor Subfamily 5, Group A, Member 1 (NR5A1))

Alternative Name: NR5A1 (NR5A1 Products)

Synonyms: AD4BP antibody, ELP antibody, FTZ1 antibody, FTZF1 antibody, POF7 antibody, SF-1 antibody, SF1 antibody, SPGF8 antibody, SRXY3 antibody, Ad4BP antibody, ELP-3 antibody, Ftz-F1 antibody, Ftzf1 antibody, Ad4BP/SF-1 antibody, Ad4bp antibody, Sf-1 antibody, NR5A1 antibody, sf-1 antibody, elp antibody, sf1 antibody, ftz1 antibody, ad4bp antibody, ftzf1 antibody, SF-1/Ad4BP antibody, SI:zC167P9.2 antibody, ff1d antibody, nr5a2l antibody, nuclear receptor subfamily 5 group A member 1 antibody, nuclear receptor subfamily 5, group A, member 1 antibody, nuclear receptor subfamily 5 group A member 1 L homeolog antibody, nuclear receptor subfamily 6 group A member 1 antibody, nuclear receptor subfamily 5, group A, member 1b antibody, NR5A1 antibody, Nr5a1 antibody, nr5a1.L antibody, nr5a1 antibody, NR6A1 antibody, nr5a1b antibody

Background: Adrenal 4 binding protein (AD4BP), ELP, Fushi tarazu factor homolog 1 (FTZ1), Nuclear receptor AdBP4, Nuclear receptor subfamily 5 group A member 1 (NR5A1), POF7, SPGF8, SRXY3, Steroid hormone receptor Ad4BP, Steroidogenic factor 1 (SF1), Steroidogenic factor 1 nuclear receptor, STF1,Steroidogenic Factor 1 (SF-1) (Transcription Factor)
Cellular localisation: Nucleus.

Molecular Weight: 53kDa

Gene ID: 2516, 495108

UniProt: Q13285

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Maintenance of Protein Location