GLUL antibody (AA 50-250)

Catalog No. ABIN7126480

Product Details anti-GLUL Antibody

Target: GLUL (Glutamate-Ammonia Ligase (GLUL))

Binding Specificity: AA 50-250

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GLUL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: Glutamine synthetase (Gl Syn) forms a homo-octamer that serves as a catalyst for the amination of glutamic acid to form glutamine. This enzyme is a marker for astrocytes, which serve as the primary site of conversion of glutamic acid to glutamine in the brain. Induction of glutamine synthetase is seen upon astrocyte cell contact with neurons. Elevated expression of glutamine synthetase in glial cells has been shown to protect neurons from degeneration due to excess glutamate. Glutamine synthetase is also present in the liver and is involved in nitrogen homeostasis. Overexpression of glutamine synthetase has been shown in primary liver cancers, indicating a potential role for glutamine synthetase in hepatocyte transformation.

Cross-Reactivity (Details): Human. Horse. Cow.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa 50-250) of human GLUL protein (exact sequence is proprietary)

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human brain, stomach or thyroid. A-431 or Y79 cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for GLUL

Target: GLUL (Glutamate-Ammonia Ligase (GLUL))

Alternative Name: GLUL (GLUL Products)

Synonyms: GLNS antibody, GS antibody, PIG43 antibody, PIG59 antibody, Glns antibody, glns antibody, glul antibody, pig43 antibody, pig59 antibody, xgs antibody, p42 antibody, MGC64262 antibody, cb195 antibody, cb757 antibody, etID49849.2 antibody, fb39g09 antibody, fd19d09 antibody, gso1 antibody, sb:cb195 antibody, wu:fb39g09 antibody, wu:fd19d09 antibody, fb11d08 antibody, gso2 antibody, hm:zehn1949 antibody, im:7153900 antibody, wu:fb11d08 antibody, glutamate-ammonia ligase antibody, glutamate-ammonia ligase (glutamine synthetase) antibody, type I glutamate--ammonia ligase antibody, glutamate-ammonia ligase L homeolog antibody, glutamate-ammonia ligase (glutamine synthase) antibody, glutamate-ammonia ligase (glutamine synthase) a antibody, glutamate-ammonia ligase (glutamine synthase) b antibody, GLUL antibody, Glul antibody, AMF_RS09530 antibody, glul.L antibody, glul antibody, CNA04370 antibody, glula antibody, glulb antibody

Background: Cell proliferation-inducing protein 59, GLUL, Glutamate ammonia ligase, GS, PIG43, PIG59, Glutamate decarboxylase, glutamine synthase, Glutamine synthetase, glutamine synthetase I, Proliferation inducing protein 43, GLNA, GLNS,Glutamine Synthetase / GLUL
Cellular localisation: Cytoplasm. Mitochondrion.

Molecular Weight: 42kDa

Gene ID: 2752, 518525

UniProt: P15104

Pathways: Positive Regulation of Peptide Hormone Secretion