Recombinant c-MYC antibody (AA 408-439)

Catalog No. ABIN7126731

The Mouse Monoclonal anti-c-MYC antibody has been validated for IHC (f). It is suitable to detect c-MYC in samples from Human.

Quick Overview for Recombinant c-MYC antibody (AA 408-439) (ABIN7126731)

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This c-MYC antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-c-MYC Antibody

Binding Specificity: AA 408-439

Specificity: It recognizes a transcription factor of 64-67 kDa, identified as c-myc. Its epitope spans between aa 410-419 (EQKLISEEDL) which is a specific portion of an alpha helical region of human c-myc protein. This MAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

Cross-Reactivity (Details): Human. Does not react with mouse, rat, and chicken.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: A synthetic peptide, corresponding to aa 408-439 (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) from C-terminus of human c-myc, coupled to KLH.

Isotype: IgG1

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HL-60 cells or Cervical Carcinoma

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for c-MYC

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Alternative Name: MYC

Background: Class E basic helix-loop-helix protein 39 (bHLHe39), MRTL, Myc2, Niard, Nird, Proto-oncogene c-Myc, RNCMYC, Transcription factor p64, Transcriptional regulator Myc-A, V-Myc avian myelocytomatosis viral oncogene homolog,c-Myc Oncoprotein
Cellular localisation: Nuclear

Molecular Weight: 62-64kDa

Gene ID: 4609, 202453

UniProt: P01106

Pathways: p53 Signaling, Cell Division Cycle, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Warburg Effect