PSMD4/ASF antibody

Catalog No. ABIN7126850

Product Details anti-PSMD4/ASF Antibody

Target: PSMD4/ASF (Psmd4) (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 4 (Psmd4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PSMD4/ASF antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: In eukaryotic cells, selective breakdown of cellular proteins is ensured by two distinct pathways. First, appropriate proteins are tagged for degradation by ubiquitination. Second, these multiubiquitinated proteins are degraded by the highly selective 26S Proteasome protein-destroying machinery. At specific stages of development, embryo- and tissue-specific components of the 26S Proteasome are formed, which are termed Rpn10a through Rpn10e, or alternatively pUb-R2 through pUb-R5. All members of this family can be generated by a single Rpn10 gene by developmentally regulated alternative splicing. The pUb-R2 subunit, originally identified as S5a (also designated antisecretory factor and multiubiquitin chain binding protein) is ubiquitously expressed and may perform proteolysis constitutively in a wide variety of cells. pUb-R4 and pUb-R5 may have embryo- or tissue-specific expression and may play specialized roles in early embryonic development.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human PSMD4protein

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: Jurkat or HepG2 cells. Liver tissue lysate. Human liver, tonsil or kidney.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for PSMD4/ASF

Target: PSMD4/ASF (Psmd4) (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 4 (Psmd4))

Alternative Name: PSMD4 (Psmd4 Products)

Synonyms: af-1 antibody, mcb1 antibody, pub-r5 antibody, rpn10 antibody, PSMD4 antibody, DDBDRAFT_0202512 antibody, DDBDRAFT_0232981 antibody, DDB_0202512 antibody, DDB_0232981 antibody, AF antibody, AF-1 antibody, ASF antibody, MCB1 antibody, Rpn10 antibody, S5A antibody, pUB-R5 antibody, xrpn10c antibody, Af1 antibody, Mcb1 antibody, angiocidin antibody, im:6912672 antibody, psmd4 antibody, wu:fc68c04 antibody, zgc:110247 antibody, proteasome 26S subunit, non-ATPase 4 antibody, 26S proteasome non-ATPase regulatory subunit 4 antibody, 26s proteasome non-atpase regulatory subunit 4 (26s proteasomeregulatory subunit s5a) antibody, proteasome 26S subunit, non-ATPase 4 L homeolog antibody, proteasome (prosome, macropain) 26S subunit, non-ATPase, 4 antibody, proteasome 26S subunit, non-ATPase 4b antibody, psmd4 antibody, PSMD4 antibody, LOC100194679 antibody, psmD4 antibody, LOC100283637 antibody, Smp_000740.1 antibody, psmd4.L antibody, Psmd4 antibody, psmd4b antibody

Background: 26S proteasome non-ATPase regulatory subunit 4, 26S proteasome regulatory subunit RPN10, 26S proteasome regulatory subunit S5A, AF, AF-1,26S proteasome non-ATPase regulatory subunit 4
Cellular localisation: Nucleus and cytoplasm.

Molecular Weight: 50kDa

Gene ID: 5710, 505059

UniProt: P55036

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Ubiquitin Proteasome Pathway