RAC1 antibody

Catalog No. ABIN7126866

Product Details anti-RAC1 Antibody

Target: RAC1

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RAC1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages (By similarity). Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Rac1 has been demonstrated to play a critical role in tumor progression of human colorectal adenocarcinoma cells. In one study, overexpression of Rac1 accelerated the tumorigenic process, whereas Rac1 inhibition completed suppressed tumor formation.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human full-length RAC1protein

Isotype: IgG1

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or Jurkat cells. Human breast, prostate or tonsil tissues.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for RAC1

Target: RAC1

Abstract: RAC1 Products

Synonyms: Rac-1 antibody, TC-25 antibody, p21-Rac1 antibody, AL023026 antibody, D5Ertd559e antibody, RAC2 antibody, CRAC1A antibody, rac1 antibody, wu:fd16e02 antibody, zgc:55823 antibody, zgc:55917 antibody, zgc:86934 antibody, 2248 antibody, CG2248 antibody, D-Rac antibody, D-Rac 1 antibody, D-Rac1 antibody, DRac antibody, DRac1 antibody, DRacA antibody, Dm Rac1 antibody, DmRAC antibody, DmRAC1 antibody, Dmel\CG2248 antibody, Dmrac1 antibody, Drac antibody, Drac1 antibody, Drac1a antibody, DracA antibody, GTPase Rac1 antibody, P40792 antibody, RAC1 antibody, Rac antibody, Rac GTPase antibody, Rac3 antibody, RacA antibody, dRac antibody, dRac1 antibody, drac1 antibody, rac antibody, Xrac antibody, mig5 antibody, p21-rac1 antibody, tc-25 antibody, Rac family small GTPase 1 antibody, RAS-related C3 botulinum substrate 1 antibody, ras-related C3 botulinum toxin substrate 1 antibody, ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) antibody, Rac family small GTPase 1a antibody, CG2248 gene product from transcript CG2248-RB antibody, Rac family small GTPase 1 L homeolog antibody, ras homolog family member G antibody, RAC1 antibody, Rac1 antibody, rac1a antibody, rac1.L antibody, RHOG antibody

Background: Cell migration-inducing gene 5 protein, MIG5, Rac-1, Ras-like protein TC25, Ras-related C3 botulinum toxin substrate 1,Ras-related C3 botulinum toxin substrate 1
Cellular localisation: Cell surface. Melanosome.

Molecular Weight: 21kDa

Gene ID: 5879, 413812

UniProt: P63000

Pathways: WNT Signaling, Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Thromboxane A2 Receptor Signaling, VEGF Signaling