Recombinant Cyclin B1 antibody
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- Target See all Cyclin B1 (CCNB1) Antibodies
- Cyclin B1 (CCNB1)
- Antibody Type
- Recombinant Antibody
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Monoclonal
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Conjugate
- This Cyclin B1 antibody is un-conjugated
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Application
- Positive Control (PC)
- Specificity
- It recognizes a protein of 55-62 kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.
- Cross-Reactivity (Details)
- Human, Mouse and Hamster.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant full-length human CCNB1 protein
- Isotype
- IgG
- Top Product
- Discover our top product CCNB1 Primary Antibody
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- Application Notes
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Known_Application: Optimal dilution for a specific application should be determined.
Positive_Control: Cell line in logarithmic growth phase. Human tonsil, prostate or ovarian carcinoma.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- Cyclin B1 (CCNB1)
- Alternative Name
- CCNB1 (CCNB1 Products)
- Synonyms
- ccnb antibody, cycb antibody, cb267 antibody, cycb1 antibody, wu:fa19g04 antibody, wu:fb16d01 antibody, wu:fb16e07 antibody, wu:fi21c01 antibody, ccnb1 antibody, MGC53596 antibody, CCNB antibody, Ccnb1-rs1 antibody, Ccnb1-rs13 antibody, CycB1 antibody, Cycb-4 antibody, Cycb-5 antibody, Cycb1-rs1 antibody, cyclin B1 antibody, cyclin B1 S homeolog antibody, ccnb1 antibody, ccnb1.2 antibody, ccnb1.S antibody, CCNB1 antibody, Ccnb1 antibody, ccnb1.2.S antibody
- Background
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CCNB, CCNB1, CCNB1_HUMAN, G2 Mitotic Specific Cyclin B1,Cyclin B1 (G2- & M-phase Cyclin)
Cellular localisation: Cytoplasm. - Molecular Weight
- 55-62kDa
- Gene ID
- 891, 23960
- UniProt
- P14635
- Pathways
- Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase
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