QKI antibody

Catalog No. ABIN7127192

Product Details anti-QKI Antibody

Target: QKI (Protein Quaking (QKI))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This QKI antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS)

Specificity: QKI, also known as HKQ, QK, QK3 or quaking, is a 341 amino acid protein that localizes to both the cytoplasm and the nucleus and contains one KH domain. Expressed in the frontal cortex of the brain, QKI functions as an RNA-binding protein that plays an important role in myelinization and specifically binds to the RNA core sequence 5'-NACUAAY-N(1,20)-UAAY-3'. Additionally, QKI regulates pre-mRNA splicing, and mRNA export and is involved in protecting and promoting the stability of select mRNAs. QKI may be methylated by PRMT1 and may also be phosphorylated at its C-terminus, an event that decreases QKI mRNA-binding affinity. Defects or deletions in the gene encoding QKI are associated with astrocytic tumors and may be involved in the pathogenesis of schizophrenia. Multiple isoforms of QKI exist due to alternative splicing events.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human QKI protein

Isotype: IgG2

Application Details

Application Notes:

Known_Application: ELISA (concentration assay dependent), Flow Cytometry (1-2 μg/million cells),Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: U87, HeLa, K562 or MCF7 cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for QKI

Target: QKI (Protein Quaking (QKI))

Alternative Name: QKI (QKI Products)

Synonyms: Hqk antibody, QK antibody, QK1 antibody, QK3 antibody, hqkI antibody, 1110003F05Rik antibody, 1500005P18 antibody, QkI antibody, l(17)-1Wis antibody, l17Wis1 antibody, Qki antibody, CQKI antibody, QKI-5 antibody, PqkI antibody, BqkI antibody, qk antibody, qk1 antibody, quaking antibody, Xqua antibody, qki antibody, EQKI antibody, EqkI antibody, QKI, KH domain containing RNA binding antibody, quaking antibody, QKI, KH domain containing, RNA binding S homeolog antibody, QKI, KH domain containing, RNA binding L homeolog antibody, QKI antibody, Qk antibody, qki.S antibody, qki.L antibody, Qki antibody

Background: Homolog of mouse quaking QKI KH domain RNA binding protein, HQK1, Protein quaking, QK1, QK3, QKI, QKI1, Quaking homolog KH domain RNA binding mouse, Quaking isoform 1, Quaking protein, RNA binding protein HQK,QKI / Protein Quaking
Cellular localisation: Nucleus. Cytoplasm.

Molecular Weight: 37.6kDa

Gene ID: 9444, 510324

UniProt: Q96PU8