Recombinant EZH2 antibody
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- Target See all EZH2 Antibodies
- EZH2 (Enhancer of Zeste Homolog 2 (EZH2))
- Antibody Type
- Recombinant Antibody
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Monoclonal
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Conjugate
- This EZH2 antibody is un-conjugated
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Application
- ELISA, Immunohistochemistry (IHC)
- Cross-Reactivity
- Human
- Purification
- Affinity-chromatography
- Immunogen
- A synthesized peptide derived from human KMT6 / EZH2
- Clone
- 10H8
- Isotype
- IgG
- Top Product
- Discover our top product EZH2 Primary Antibody
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- Application Notes
- Recommended dilution: IHC:1:50-1:200,
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C,-80 °C
- Storage Comment
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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- Target
- EZH2 (Enhancer of Zeste Homolog 2 (EZH2))
- Alternative Name
- EZH2 (EZH2 Products)
- Synonyms
- ENX-1 antibody, ENX1 antibody, EZH1 antibody, EZH2b antibody, KMT6 antibody, KMT6A antibody, WVS antibody, WVS2 antibody, eya2 antibody, im:7149338 antibody, zgc:136376 antibody, zgc:152758 antibody, EZH2 antibody, enx-1 antibody, enx1 antibody, ezh1 antibody, kmt6 antibody, xez antibody, Enx-1 antibody, Enx1h antibody, mKIAA4065 antibody, ezh2 antibody, olezh2 antibody, enhancer of zeste 2 polycomb repressive complex 2 subunit antibody, enhancer of zeste 2 polycomb repressive complex 2 subunit L homeolog antibody, enhancer of zeste 2 polycomb repressive complex 2 subunit S homeolog antibody, EZH2 antibody, ezh2 antibody, Ezh2 antibody, ezh2.L antibody, ezh2.S antibody
- Background
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Background: Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Displays a preference for substrates with less methylation, loses activity when progressively more methyl groups are incorporated into H3K27, H3K27me0 > H3K27me1 > H3K27me2 (PubMed:22323599). Compared to EZH1-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-ARNTL/BMAL1 heterodimer, involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription.
Aliases: Histone-lysine N-methyltransferase EZH2 (EC 2.1.1.43) (ENX-1) (Enhancer of zeste homolog 2) (Lysine N-methyltransferase 6), EZH2, KMT6
- UniProt
- Q15910
- Pathways
- Retinoic Acid Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation
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