BAD antibody (pSer155)

Catalog No. ABIN7138438

This anti-BAD antibody is a Rabbit Polyclonal antibody detecting BAD in WB, ELISA and IHC. Suitable for Mouse.

Quick Overview for BAD antibody (pSer155) (ABIN7138438)

Target: BAD (BCL2-Associated Agonist of Cell Death (BAD))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAD antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-BAD Antibody

Binding Specificity: pSer155

Purpose: Phospho-Bad (Ser155) Antibody

Cross-Reactivity: Human, Rat

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of serine 155(R-M-S(p)-D-E) derived from Human BAD.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for BAD

Target: BAD (BCL2-Associated Agonist of Cell Death (BAD))

Alternative Name: Bad

Background:

Background:

 Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

Schurmann A., Mooney A.F., Sanders L.C., Sells M.A., Wang H.G., Reed J.C., Bokoch G.M.Mol. Cell. Biol. 20:453-461(2000)

Jakobi R., Moertl E., Koeppel M.A.J. Biol. Chem. 276:16624-16634(2001)        

Aliases: Bad antibody; Bbc6Bcl2-associated agonist of cell death antibody; BAD antibody; Bcl-2-binding component 6 antibody; Bcl-xL/Bcl-2-associated death promoter antibody; Bcl2 antagonist of cell death antibody

UniProt: Q61337

Pathways: MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, Apoptosis, Fc-epsilon Receptor Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events