IRF3 antibody (pSer386)
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- Target See all IRF3 Antibodies
- IRF3 (Interferon Regulatory Factor 3 (IRF3))
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Binding Specificity
- pSer386
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This IRF3 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA
- Cross-Reactivity
- Human
- Purification
- Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
- Immunogen
- Peptide sequence around phosphorylation site of Serine 386(A-S-S(p)-L-E) derived from Human IRF-3.
- Isotype
- IgG
- Top Product
- Discover our top product IRF3 Primary Antibody
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- Application Notes
- WB:1:500-1:1000,
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C,-80 °C
- Storage Comment
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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- Target
- IRF3 (Interferon Regulatory Factor 3 (IRF3))
- Alternative Name
- IRF3 (IRF3 Products)
- Synonyms
- irf3 antibody, IRF3 antibody, C920001K05Rik antibody, IRF-3 antibody, interferon regulatory factor 3 antibody, interferon regulatory factor 7 antibody, irf3 antibody, IRF3 antibody, irf-3 antibody, Irf3 antibody, IRF7 antibody
- Background
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Background:
Key transcriptional regulator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.
Au W.W.-C., Proc. Natl. Acad. Sci. U.S.A. 92:11657-11661(1995).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Bellingham J., Ann. Hum. Genet. 62:231-234(1998).Aliases: IIAE7 antibody, Interferon regulatory factor 3 antibody, IRF 3 antibody, IRF-3 antibody, IRF3 antibody, IRF3_HUMAN antibody, MGC94729 antibody
- UniProt
- Q14653
- Pathways
- TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades
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