WEE1 antibody (pSer642)

Catalog No. ABIN7138671

Product Details anti-WEE1 Antibody

Target: WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

Binding Specificity: pSer642

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WEE1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity: Human, Mouse, Rat

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of Serine 642(S-V-S(p)-L-T) derived from Human WEE1.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:1000,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for WEE1

Target: WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

Alternative Name: WEE1 (WEE1 Products)

Synonyms: WEE1A antibody, WEE1hu antibody, ATWEE1 antibody, F22D16.3 antibody, F22D16_3 antibody, WEE1 kinase homolog antibody, WEE1-KINASE antibody, WEE1 antibody, Xe-Wee1B antibody, XeWee1B antibody, wee1-b antibody, wee1a antibody, wee2-a antibody, xe-wee1a antibody, xwee1 antibody, wee1 antibody, wee1b antibody, wee1hu antibody, xwee2 antibody, GB19689 antibody, Wee1A antibody, fa20h03 antibody, wu:fa20h03 antibody, zgc:136514 antibody, WEE1 G2 checkpoint kinase antibody, Wee1 kinase antibody, WEE1-like kinase antibody, WEE1 G2 checkpoint kinase S homeolog antibody, wee1 homolog antibody, WEE1 homolog 2 antibody, wee1-like protein kinase antibody, WEE 1 homolog 1 (S. pombe) antibody, M phase inhibitor protein kinase Wee1 antibody, WEE1 antibody, Wee1 antibody, wee1.S antibody, wee1-a antibody, wee2 antibody, LOC551677 antibody, wee1 antibody

Background:

Background:

Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.

Watanabe N., EMBO J. 14:1878-1891(1995).
Cichutek A., Cytogenet. Cell Genet. 93:277-283(2001).
Igarashi M., Nature 353:80-83(1991)

Aliases: DKFZp686I18166 antibody, EC 2.7.10.2 antibody, FLJ16446 antibody, MGC105683 antibody, OTTHUMP00000231338 antibody, OTTHUMP00000231339 antibody, Wee 1 antibody, WEE 1 homolog 1 (S. pombe) antibody, WEE1 antibody, WEE1 homolog (S. pombe) antibody, Wee1 homolog antibody, WEE1 homolog S. pombe antibody, Wee1 like protein kinase antibody, Wee1 tyrosine kinase antibody, Wee1+ homolog antibody, Wee1+ S. pombe homolog antibody, WEE1, S. pombe, homolog of antibody, WEE1, somatic antibody, Wee1-like protein kinase antibody, WEE1_HUMAN antibody, WEE1A antibody, Wee1A kinase antibody, WEE1hu antibody

UniProt: P30291

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase