RASGRF1 antibody (pSer929) (Biotin)

Catalog No. ABIN713203

This Rabbit Polyclonal antibody specifically detects RASGRF1 in ELISA, WB, IHC (p) and IHC (fro). It exhibits reactivity toward Rat.

Quick Overview for RASGRF1 antibody (pSer929) (Biotin) (ABIN713203)

Target: RASGRF1 (Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RASGRF1 antibody is conjugated to Biotin

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-RASGRF1 Antibody (Biotin)

Binding Specificity: pSer929

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Dog,Cow,Sheep,Pig,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human RasGRF1 around the phosphorylation site of Ser929

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for RASGRF1

Target: RASGRF1 (Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1))

Alternative Name: RASGRF1

Background:

Synonyms: RASGRF1phospho S929, RASGRF1phospho Ser929, p-RASGRF1S929, p-RASGRF1Ser929, CDC25, CDC25L, GNRP, GRF1, GRF55, Guanine nucleotide exchange factor, Guanine nucleotide releasing factor 55 kD, Guanine nucleotide releasing protein, HGRF55, PP13187, Ras protein specic guanine nucleotide releasing factor 1, Ras specic guanine nucleotide releasing factor, Ras specic guanine nucleotide releasing factor CDC25 homolog, Ras specic nucleotide exchange factor CDC25.

Background: The protein encoded by this gene is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Mar 2009]

Gene ID: 5923

Pathways: RTK Signaling, Cell Division Cycle, M Phase