BAD antibody (Cleaved-Asp71)

Catalog No. ABIN7212460

The Rabbit Polyclonal anti-BAD antibody has been validated for WB, ELISA and IHC (p). It is suitable to detect BAD in samples from Mouse and Rat.

Quick Overview for BAD antibody (Cleaved-Asp71) (ABIN7212460)

Target: BAD (BCL2-Associated Agonist of Cell Death (BAD))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAD antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-BAD Antibody

Binding Specificity: AA 40-120, Cleaved-Asp71

Purpose: Cleaved-Bad (D71) Polyclonal Antibody

Specificity: Cleaved-Bad (D71) Polyclonal Antibody detects endogenous levels of fragment of activated Bad protein resulting from cleavage adjacent to D71.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of mouse Bad at AA range: 40-120

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for BAD

Target: BAD (BCL2-Associated Agonist of Cell Death (BAD))

Alternative Name: Bad

Background: Rabbit Anti-Cleaved-Bad (D71) Polyclonal Antibody,BAD, BBC6, BCL2L8, Bcl2 antagonist of cell death, BAD, Bcl-2-binding component 6, Bcl-2-like protein 8, Bcl2-L-8, Bcl-XL/Bcl-2-associated death promoter,BCL2 associated agonist of cell death encoded by BAD is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. BCL2 associated agonist of cell death positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of BCL2 associated agonist of cell death is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of BCL2 associated agonist of cell death. Alternative splicing of BAD results in two transcript variants which encode the same isoform.,Bcl2 antagonist of cell death

Molecular Weight: observerd band 20kDa

Gene ID: 572

UniProt: Q92934

Pathways: MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, Apoptosis, Fc-epsilon Receptor Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events