DDR1 antibody (pTyr513)

Catalog No. ABIN7213284

The Rabbit Polyclonal anti-DDR1 antibody is suitable to detect DDR1 in samples from Human, Rat and Mouse. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for DDR1 antibody (pTyr513) (ABIN7213284)

Target: DDR1 (Discoidin Domain Receptor tyrosine Kinase 1 (DDR1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDR1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-DDR1 Antibody

Binding Specificity: pTyr513

Purpose: Rabbit Anti-DDR1 (phospho Tyr513) Polyclonal Antibody

Specificity: Phospho-DDR1 (Y513) Polyclonal Antibody detects endogenous levels of DDR1 protein only when phosphorylated at Y513.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human DDR1 Phospho-Tyr513

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for DDR1

Target: DDR1 (Discoidin Domain Receptor tyrosine Kinase 1 (DDR1))

Alternative Name: DDR1

Background: DDR1, CAK, EDDR1, NEP, NTRK4, PTK3A, RTK6, TRKE, Epithelial discoidin domain-containing receptor 1, Epithelial discoidin domain receptor 1, CD167 antigen-like family member A, Cell adhesion kinase, Discoidin receptor tyrosine kinase, HGK2,Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases are involved in the regulation of cell growth, differentiation and metabolism.Discoidin domain receptor tyrosine kinase 1 encoded by DDR1 belongs to a subfamily of tyrosine kinase receptors with homology to Dictyostelium discoideum protein discoidin I in their extracellular domain, and that are activated by various types of collagen. Expression of this protein is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. Alternatively spliced transcript variants encoding different isoforms have been described for DDR1.

Molecular Weight: 110kD

Gene ID: 780

UniProt: Q08345

Pathways: RTK Signaling, Smooth Muscle Cell Migration