GCKR antibody (AA 180-260)

Catalog No. ABIN7221014

The Rabbit Polyclonal anti-GCKR antibody is suitable to detect GCKR in samples from Human, Rat and Mouse. It has been validated for WB and ELISA.

Quick Overview for GCKR antibody (AA 180-260) (ABIN7221014)

Target: GCKR (Glucokinase (Hexokinase 4) Regulator (GCKR))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCKR antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-GCKR Antibody

Binding Specificity: AA 180-260

Purpose: Rabbit Anti-GCKR Polyclonal Antibody

Specificity: GCKR Polyclonal Antibody detects endogenous levels of GCKR protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human GCKR at AA range: 180-260

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:40000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for GCKR

Target: GCKR (Glucokinase (Hexokinase 4) Regulator (GCKR))

Alternative Name: GCKR

Background: GCKR, Glucokinase regulatory protein, Glucokinase regulatorGCKR encodes glucokinase regulator belonging to the GCKR subfamily of the SIS (Sugar ISomerase) family of proteins. The gene product is a regulatory protein that inhibits glucokinase in liver and pancreatic islet cells by binding non-covalently to form an inactive complex with the enzyme. GCKR is considered a susceptibility gene candidate for a form of maturity-onset diabetes of the young (MODY).

Molecular Weight: 68kD

Gene ID: 2646

UniProt: Q14397

Pathways: Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus