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HSP27 antibody (pSer82)

Name: HSP27 antibody (pSer82)
Brand: antibodies-online
SKU: ABIN7211454
Price: 484.64 USD
Availability: InStock

HSPB1 Reactivity: Human, Rat, Mouse, Sheep, Chicken, Dog, Cow, Guinea Pig WB, IF, IP Host: Rabbit Polyclonal unconjugated

Western blot analysis of phosphorylated HSP27 and nonphosphorylated HSP27 proteins probed with HSP27 (pSer82) pAb (HSP27 antibody (pSer82))

Immunofluorescent analysis (confocal) staining of HeLa cells using Hsp27 (pSer82) pAb (green), nuclei are stained in blue pseudocolor using DRAQ5 (HSP27 antibody (pSer82))

2 images

Catalog No. ABIN7211454

Datasheet as PDF

Target See all HSP27 (HSPB1) Antibodies

HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
Binding Specificity
36

35

26

16

16

9

7

7

7

5

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pSer82
Reactivity
284

101

87

24

13

13

13

10

9

5

4

4

3

2

2

1

1
Human, Rat, Mouse, Sheep, Chicken, Dog, Cow, Guinea Pig
Host
225

63
Rabbit
Compatible Secondaries
Clonality
201

86
Polyclonal
Conjugate
126

23

16

13

11

7

4

4

4

4

4

4

4

4

4

4

4

4

4

3

3

3

3

3

3

3

3

3

3

2

1

1

1

1

1

1

1

1
This HSP27 antibody is un-conjugated
Application
244

103

98

94

65

47

46

41

41

40

15

12

10

7

7

2

2

2

2

2

2

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Characteristics

HspB1| Heat shock protein 27,HSP 27 (pSer82) polyclonal antibody

Purification

Whole rabbit serum.

Immunogen

Synthetic peptide corresponding to a portion of human HSP27 phosphorylated at Ser82.

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anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 101-205) antibody
HSPB1 Reactivity: Human, Rat, Mouse WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody
HSPB1 Reactivity: Human, Rat WB, IHC, ELISA, FACS, ICC Host: Mouse Monoclonal 5D7 unconjugated

anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody
HSPB1 Reactivity: Human WB, IHC, ELISA, IF, FACS, IP Host: Rabbit Monoclonal 10C11 unconjugated Recombinant Antibody

anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody
HSPB1 Reactivity: Human WB, IHC, IF, FACS, IP, ICC Host: Mouse Monoclonal 8A7 unconjugated

anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody
HSPB1 Reactivity: Human, Rat, Mouse, Sheep, Monkey, Chicken, Chimpanzee WB, IHC, IF, FACS, ISt, StM Host: Mouse Monoclonal G3-1 unconjugated

anti-Heat Shock 27kDa Protein 1 (HSPB1) (Center) antibody
HSPB1 Reactivity: Human, Rat, Mouse WB, IF, FACS, IHC (p), IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 96-205) antibody
HSPB1 Reactivity: Human WB, ELISA, IF, IP, PLA Host: Mouse Monoclonal 3G3 unconjugated

anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody
HSPB1 Reactivity: Human WB, IHC, IF, FACS, ICC Host: Rabbit Polyclonal unconjugated

anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody
HSPB1 Reactivity: Human WB, IHC, ELISA, IF, IP, ICC Host: Mouse Monoclonal 5D12-A12 unconjugated

anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (HRP)
HSPB1 Reactivity: Human WB, IHC, IF, FACS, IP, ICC Host: Mouse Monoclonal 8A7 HRP

Application Notes

Immunoprecipitation (1:100)Western Blot (1:2,000, colorimetric)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application.,Detects a band of ~27 kDa by Western blot.

Restrictions

For Research Use only
Format

Liquid

Buffer

Liquid. Whole rabbit serum.

Handling Advice

Avoid freeze/thaw cycles.

Storage

-20 °C

Storage Comment

Serum should be stored at -20°C for long term storage. For short term storage, 4°C, supplement with 0.1% sodium azide.
Target

HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Alternative Name

HSP25, HSP27 (HSPB1 Products)

Synonyms

CMT2F antibody, HMN2B antibody, HS.76067 antibody, HSP27 antibody, HSP28 antibody, Hsp25 antibody, SRP27 antibody, 27kDa antibody, Hsp27 antibody, cb153 antibody, cb660 antibody, hsp1 antibody, hsp25 antibody, hsp27 antibody, id:ibd2821 antibody, sb:cb660 antibody, zgc:103437 antibody, HSPB1 antibody, cmt2f antibody, hs.76067 antibody, hsp28 antibody, heat shock protein family B (small) member 1 antibody, heat shock protein 1 antibody, heat shock protein, alpha-crystallin-related, 1 antibody, heat shock protein family B (small) member 1 L homeolog antibody, HSPB1 antibody, Hspb1 antibody, hspb1 antibody, hspb1.L antibody

UniProt

P04792

Pathways

MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling