Recombinant RFP antibody

Catalog No. ABIN7272904

Product Details anti-RFP Antibody

Target: RFP (Red Fluorescent Protein (RFP))

Antibody Type: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reactivity: Discosoma

Host: Alpaca

Expression System: E.coli

Clonality: Monoclonal

Conjugate: This RFP antibody is un-conjugated

Application: Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Specificity: MRFP and derivatives like mCherry, mScarlet-i, tdTomato, dsRed, mOrange

Purification: Produced in: E.coli

Immunogen: Our nanobodies bind specifically and strongly to DsRed, RFP, and mCherry, among others family members. Look at our specificity chart in the resource section.

Clone: 2B12

Application Details

Application Notes: Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconsitute with 250 µL pure water for immidate use

Buffer: sdAbs are lyohilized from 10 mM KPi pH 6.0,150 mM NaCl, 1 mM EDTA pH 6.

Storage: 4 °C/-20 °C

Storage Comment: Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below.

Target Details for RFP

Target: RFP (Red Fluorescent Protein (RFP))

Alternative Name: mRFP (RFP Products)

Background: One of the first red-emitting fluorescent proteins (FP) described was DsRed from Discosoma sp. Only shortly after its publication in 1999 and including more than 20 mutations, a monomeric version with several enhanced characteristics was discovered, mRFP1. From this promising monomeric red-FP, dozens of monomeric variants have been described, from which mCherry represents one of the most popular derivatives.