GLI3 antibody (AA 875-925)

Catalog No. ABIN7454914

Product Details anti-GLI3 Antibody

Target: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Binding Specificity: AA 875-925

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLI3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Purpose: Rabbit anti-GLI3 Antibody, Affinity Purified

Predicted Reactivity: Mouse,Chimpanzee

Purification: Affinity Purified

Immunogen: between AA 875 and 925

Isotype: IgG

Application Details

Application Notes:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for GLI3

Target: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Alternative Name: GLI3 (GLI3 Products)

Synonyms: ACLS antibody, GCPS antibody, GLI3-190 antibody, GLI3FL antibody, PAP-A antibody, PAPA antibody, PAPA1 antibody, PAPB antibody, PHS antibody, PPDIV antibody, xgli3 antibody, AI854843 antibody, AU023367 antibody, Bph antibody, Pdn antibody, Xt antibody, add antibody, im:7142603 antibody, GLI3 antibody, GLI family zinc finger 3 antibody, GLI family zinc finger 3 L homeolog antibody, GLI-Kruppel family member GLI3 antibody, GLI3 antibody, Gli3 antibody, gli3.L antibody, gli3 antibody

Background: Background: GLI3 (GLI family zinc finger 3) plays a role in limb development and has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway. After phosphorylation and nuclear translocation, the full-length form of GLI3 (GLI3FL), acts as an activator (GLI3A) while the C-terminally truncated form (GLI3R) acts as a repressor [taken from the Universal Protein Resource (UniProt) P10071].

Gene ID: 2737

NCBI Accession: NP_000159

UniProt: P10071

Pathways: Hedgehog Signaling