Recombinant p21 antibody (AA 120-138)

Catalog No. ABIN7487749

The Rabbit Chimeric anti-p21 antibody (Clone Y13-238) (ABIN7487749) specifically detects p21 in IP. The antibody is reactive with Human and Mouse samples.

Quick Overview for Recombinant p21 antibody (AA 120-138) (ABIN7487749)

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Chimeric

Conjugate: This p21 antibody is un-conjugated

Application: Immunoprecipitation (IP)

Clone: Y13-238

Product Details anti-p21 Antibody

Binding Specificity: AA 120-138

Purpose: Anti-p21 [Y13-238], Rabbit IgG, lambda

Specificity:

The antibody reacts with v-H-ras (within amino acids 120-138) and rodent c-H-ras p21's but not v-K-ras or rodent c-K-ras p21's. This antibody also recognizes human c-H-ras and c-K-ras p21's, but not human c-N-ras.

Application Notes original Clone: Y13-238 has been used to immunoprecipitate p21 from human peripheral blood lymphoblasts in order to measure its activation state (Downward 1990). Y13-238 has been used as an anti-ras non-neutralising scFv (Cochet 1998).

Cross-Reactivity: Mouse

Characteristics: This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Purification: Protein A affinity purified

Immunogen: Injection of rats subcutaneously with Ha-MuSV transformed NRK cells and screening of sera of individual animals to identify those with the strongest immune response against p21. Spleens were removed from the immunised animals and cell fusion of immune lymphocytes with rat myeloma cell line 210RCY3 Ag 1.2.3 (Y3) was carried out. Supernatants were harvested and assayed for antibodies to p21 by GDP binding assay or immunofluorescence on Ha-SV-transformed MDCK cells, followed by testing for the ability of the antibodies to immunoprecipitate p21.

Isotype: IgG, lambda

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

OriginalSpeciesName: Rat

OriginalFormat: IgG2a

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for p21

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Alternative Name: p21

Background: Ras, p21ras,

UniProt: P78460, O70564

Pathways: p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy