alpha-Thrombin antibody

Catalog No. ABIN7536660

Product Details anti-alpha-Thrombin Antibody

Target: alpha-Thrombin

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This alpha-Thrombin antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunodepletion (IDe), Immunoelectrophoresis (IEP), Immunoprecipitation (IP), Neutralization (Neut)

Purpose: Thrombin Polyclonal Antibody - Affinity Purified

Specificity: Human Thrombin, alpha-thrombin

Purification: Antigen-specific affinity purification

Immunogen: Thrombin prepared from purified human prothrombin, active site blocked with PPACK.

Isotype: IgG

Application Details

Application Notes: Immunoblot 0.5-5 μg/mL, ELISA capture 10 μg/mL

Comment:

Affinity-purified IgG are antibodies further purified using immobilized antigen. Affinity-purification is an enrichment process in which whole IgG is passed over a resin to which antigen has been covalently attached. Non-reactive antibodies are washed away and specific antibodies are eluted under conditions that disrupt the antigen-antibody interaction. The result is a very high-purity product with a substantially higher titre than whole IgG. This level of product is intended for applications such as immunoblotting, immunostaining of cells and several types of immunoassays where the higher signal-to-noise ratio achieved with this enriched product is required.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Allow product to warm to room temperature and gently mix before use

Concentration: 2 mg/mL

Buffer: 10 mM HEPES, pH 7.4, 150 mM NaC, 50 % (v/v) glycerol

Storage: -20 °C

Storage Comment: Store between -10°C and -20°C

Target Details for alpha-Thrombin

Target: alpha-Thrombin

Alternative Name: Thrombin (alpha-Thrombin Products)

Synonyms: PT antibody, RPRGL2 antibody, THPH1 antibody, coagulation factor II, thrombin antibody, F2 antibody