alpha-Thrombin antibody
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- Target See all alpha-Thrombin products
- alpha-Thrombin
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Reactivity
- Cow
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Host
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Sheep
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Clonality
- Polyclonal
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Conjugate
- This alpha-Thrombin antibody is un-conjugated
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Application
- ELISA, Western Blotting (WB), Immunoprecipitation (IP), Neutralization (Neut), Immunodepletion (IDe), Immunoelectrophoresis (IEP)
- Purpose
- Bovine Thrombin Polyclonal Antibody - Affinity Purified
- Specificity
- Human Thrombin, alpha-thrombin
- Purification
- Antigen-specific affinity purification
- Immunogen
- Thrombin prepared from purified bovine prothrombin, active site blocked with PPACK.
- Isotype
- IgG
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- Application Notes
- Immunoblot 0.5-5 μg/mL, ELISA capture 10 μg/mL
- Comment
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Affinity-purified IgG are antibodies further purified using immobilized antigen. Affinity-purification is an enrichment process in which whole IgG is passed over a resin to which antigen has been covalently attached. Non-reactive antibodies are washed away and specific antibodies are eluted under conditions that disrupt the antigen-antibody interaction. The result is a very high-purity product with a substantially higher titre than whole IgG. This level of product is intended for applications such as immunoblotting, immunostaining of cells and several types of immunoassays where the higher signal-to-noise ratio achieved with this enriched product is required.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
- Allow product to warm to room temperature and gently mix before use
- Concentration
- 2 mg/mL
- Buffer
- 10 mM HEPES, pH 7.4, 150 mM NaC, 50 % (v/v) glycerol
- Storage
- -20 °C
- Storage Comment
- Store between -10°C and -20°C
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- Target
- alpha-Thrombin
- Alternative Name
- Thrombin (alpha-Thrombin Products)
- Synonyms
- PT antibody, RPRGL2 antibody, THPH1 antibody, coagulation factor II, thrombin antibody, F2 antibody
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