Recombinant HLAG antibody

Catalog No. ABIN7543432

The Rabbit Chimeric anti-HLAG antibody (Clone mem-G-9) (ABIN7543432) specifically detects HLAG in FACS, ELISA, IHC and IF. The antibody is reactive with Human samples.

Quick Overview for Recombinant HLAG antibody (ABIN7543432)

Target: HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This HLAG antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: Mem-G-9

Product Details anti-HLAG Antibody

Purpose: Anti-HLA-G [mem-G/9], Rabbit IgG, kappa

Specificity:

This antibody binds an epitope in the alpha 1 domain of human HLA-G. It binds the native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. It is also reported to cross react with HLA-G3.

Application Notes original Clone: This antibody is not suitable for detecting denatured human HLA-G protein in a western blot. This antibody can be used for the detection of soluble HLA-G in a sample using ELISA (PMID: 10902607). This antibody can effectively detect HLA-G and HLA-G3 on K653-HLA-G1, K562-G3 transductants, M8-HLA-G1 and JEG-3 cells in a flow cytometric analysis (PMID: 12590976, 22738368). This antibody was also used for studying the expression of HLA-G on the surface of JEG-3 cells using immunofluorescence staining (PMID: 22738368). This antibody can be used for immunohistochemical analysis of cryopreserved serial sections of trophoblast tissue. This antibody is not suitable for immunohistochemical staining of paraffin sections (PMID: 12590976). This antibody was also reported to be used in isolation of HLA-G+ cells for prenatal screening (PMID: 35498932).

Characteristics: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing mice with native, properly folded, beta-2-microglubulin (b2m)-associated HLA-G.

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

OriginalSpeciesName: Mouse

OriginalFormat: IgG1

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for HLAG

Target: HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))

Alternative Name: HLA-G

Background: HLAG, HLA-6.0, HLA-G5, HLAG-6, HLA class I histocompatibility antigen alpha chain G, HLA G antigen, MHC class I antigen G, human leukocyte antigen

UniProt: P17693

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response