FOXO3 antibody (AA 312-537)

Catalog No. ABIN7601285

This anti-FOXO3 antibody is a Rabbit Polyclonal antibody detecting FOXO3 in WB and ELISA. Suitable for Human.

Quick Overview for FOXO3 antibody (AA 312-537) (ABIN7601285)

Target: FOXO3 (Forkhead Box O3 (FOXO3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXO3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-FOXO3 Antibody

Binding Specificity: AA 312-537

Purpose: Anti-FOXO3A/FOXO3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-FOXO3A/FOXO3 Antibody Picoband® (ABIN7601285). Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human FOXO3A/FOXO3 recombinant protein (Position: R312-Q537).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Anderson MJ, Viars CS, Czekay S, Cavenee WK, Arden KC (January 1998). "Cloning and characterization of three human forkhead genes that comprise an FKHR-like gene subfamily". Genomics 47 (2): 187-99. 2. Brunet A, Bonni A, Zigmond MJ, Lin MZ, Juo P, Hu LS, Anderson MJ, Arden KC, Blenis J, Greenberg ME (March 1999). "Akt promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor". Cell 96 (6): 857-68. 3. Ekoff M, Kaufmann T, Engström M, Motoyama N, Villunger A, Jönsson JI, Strasser A, Nilsson G (November 2007). "The BH3-only protein Puma plays an essential role in cytokine deprivation induced apoptosis of mast cells". Blood 110 (9): 3209-17.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for FOXO3

Target: FOXO3 (Forkhead Box O3 (FOXO3))

Alternative Name: FOXO3

Background:

Synonyms: Angiotensin-converting enzyme, ACE, Dipeptidyl carboxypeptidase I, Kininase II, CD143, Angiotensin-converting enzyme, soluble form, Ace, Dcp1

Tissue Specificity: Testis-specific isoform is expressed in spermatocytes, adult testis.

Background: Forkhead box O3, also known as FKHRL1 or FOXO3a, is a human protein encoded by the FOXO3 gene. FOXO3 belongs to the O subclass of the forkhead family of transcription factors which are characterized by a distinct fork head DNA-binding domain. It is mapped to 6q21. This protein likely functions as a trigger for apoptosis through upregulation of genes necessary for cell death, such as Bim and PUMA, or downregulation of anti-apoptotic proteins such as FLIP. In mammals FOXO3 regulates the resistance of cells to stress by inducing DNA repair and thereby may also affect organismal life span. In addition, it is thought that FOXO3 is also involved in protection from oxidative stress by upregulating antioxidants such as catalase and MnSOD.

Molecular Weight: 90 kDa

Gene ID: 2309

UniProt: O43524

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis