PPP1R13L antibody (AA 15-100) (Cy5.5)
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- Key Features
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- High-affinity anti-GFP single-domain antibody (sdAb) covalently bound to 4 % cross-linked agarose magnetic beads with 50-150 µm diameter.
- The Alpaca sdAb clone 1H1 is specific for GFP and many GFP derivatives. Capacity >4 µg GFP / 1 µL packed beads (2 µL bead slurry).
- Compatible with physiological buffers, common Lysis and Wash Buffers, and high stringency buffers.
- Target See all PPP1R13L Antibodies
- PPP1R13L (Protein Phosphatase 1, Regulatory Subunit 13 Like (PPP1R13L))
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Binding Specificity
- AA 15-100
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Reactivity
- Human, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PPP1R13L antibody is conjugated to Cy5.5
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Application
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))
- Cross-Reactivity
- Human, Rat
- Predicted Reactivity
- Mouse,Dog,Cow,Sheep,Pig,Guinea Pig
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic peptide derived from human IASPP
- Isotype
- IgG
- Top Product
- Discover our top product PPP1R13L Primary Antibody
- Product Specific Information
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Our GFP Catcher is a product based on the GFP pull-down technique used to isolate GFP fusion proteins from cell lysates. It consists of special magnetic beads (product ABIN7272855) or agarose beads (product ABIN5311508) coated with an antibody against GFP. These beads allow efficient and selective binding to GFP or GFP-fused target proteins.
The application of GFP Catcher is similar to the GFP pull-down method. First, the protein of interest is expressed in the cells with it fused to GFP. Then, the cell lysate is prepared to extract the target proteins. The GFP catcher beads are then added to the cell lysate and bound to these proteins by specifically binding to the GFP or GFP fusion protein.
After the beads with the bound target proteins are isolated, thorough purification is performed to remove components that are not specifically bound. Finally, the target proteins can be separated from the beads and used for further analysis such as immunoblotting or mass spectrometry.
GFP Catcher offers the advantage of quick and easy isolation of GFP fusion proteins. It is a useful tool for protein analysis and allows efficient identification of proteins interacting with GFP fusion protein. GFP Catcher is widely used in cell biology research to study protein-protein interactions and deepen the understanding of cellular processes.
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- Application Notes
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FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- PPP1R13L (Protein Phosphatase 1, Regulatory Subunit 13 Like (PPP1R13L))
- Alternative Name
- IASPP (PPP1R13L Products)
- Synonyms
- AV260376 antibody, IASPP antibody, Nkip1 antibody, wa3 antibody, zgc:113058 antibody, NKIP1 antibody, RAI antibody, RAI4 antibody, protein phosphatase 1, regulatory (inhibitor) subunit 13 like antibody, protein phosphatase 1, regulatory subunit 13 like antibody, protein phosphatase 1 regulatory subunit 13 like antibody, protein phosphatase 1 regulatory subunit 13 like L homeolog antibody, Ppp1r13l antibody, ppp1r13l antibody, PPP1R13L antibody, ppp1r13l.L antibody
- Background
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Synonyms: RAI, RAI4, IASPP, NKIP1, RelA-associated inhibitor, Inhibitor of ASPP protein, Protein iASPP, NFkB-interacting protein 1, PPP1R13B-like protein, PPP1R13L, PPP1R13BL
Background: Regulator that plays a central role in regulation of apoptosis and transcription via its interaction with NF-kappa-B and p53/TP53 proteins. Blocks transcription of HIV-1 virus by inhibiting the action of both NF-kappa-B and SP1. Also inhibits p53/TP53 function, possibly by preventing the association between p53/TP53 and ASPP1 or ASPP2, and therefore suppressing the subsequent activation of apoptosis.
- Gene ID
- 10848
- UniProt
- Q8WUF5
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