BRAF antibody

Catalog No. ABIN7635253

This Rabbit Polyclonal antibody specifically detects BRAF in IF and ICC. It exhibits reactivity toward Human.

Quick Overview for BRAF antibody (ABIN7635253)

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRAF antibody is un-conjugated

Application: Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-BRAF Antibody

Purpose: Polyclonal Antibody to B-Raf Proto Oncogene Serine/Threonine Protein Kinase (BRAF)

Specificity: The antibody is a rabbit polyclonal antibody raised against BRAF. It has been selected for its ability to recognize BRAF in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPG347Hu01Recombinant BRaf Proto Oncogene Serine/Threonine Protein Kinase (BRAF)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for BRAF

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Alternative Name: BRAF

Background: B-raf 1, BRAF1, RAFB1, V-Raf Murine Sarcoma Viral Oncogene Homolog B1

UniProt: P15056

Pathways: MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy