PDK4 antibody

Catalog No. ABIN7645232

The Rabbit Polyclonal anti-PDK4 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect PDK4 in samples from Human.

Quick Overview for PDK4 antibody (ABIN7645232)

Target: PDK4 (Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDK4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-PDK4 Antibody

Purpose: Polyclonal Antibody to Pyruvate Dehydrogenase Kinase 4 (PDK4)

Specificity: The antibody is a rabbit polyclonal antibody raised against PDK4. It has been selected for its ability to recognize PDK4 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPA958Hu01Recombinant Pyruvate Dehydrogenase Kinase 4 (PDK4)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mLImmunohistochemistry: 5-20 μg/mLImmunocytochemistry: 5-20 μg/mLOptimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.4 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: ProClin, Sodium azide

Precaution of Use: This product contains ProClin and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Target Details for PDK4

Target: PDK4 (Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4))

Alternative Name: PDK4

Background: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial, Pyruvate Dehydrogenase Kinase Isozyme 4

UniProt: Q16654

Pathways: PI3K-Akt Signaling, Hedgehog Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect