Recombinant c-MET antibody (C-Term)

Catalog No. ABIN7795683

The Rabbit Monoclonal anti-c-MET antibody is suitable to detect c-MET in samples from Human. It has been validated for WB, IHC, FACS, ICC and IP.

Quick Overview for Recombinant c-MET antibody (C-Term) (ABIN7795683)

Target: c-MET (MET) (Met Proto-Oncogene (MET))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This c-MET antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone: BLR283L

Product Details anti-c-MET Antibody

Binding Specificity: C-Term

Purpose: Rabbit anti-Met Recombinant Monoclonal Antibody [BLR283L]

Purification: Purified

Immunogen: Between 1340 and C-terminus

Isotype: IgG

Application Details

Application Notes: Flow-Cyt,Fixed in 4 % formaldehyde and permeabilized with 90 % methanol. 0.5 μL per 1 x 10^6 cells.,ICC,1100 to 1500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.,IHC,1100 to 1500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.,IP,6 μL/mg lysate ,WB,11000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1000 μg/mL

Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.09 % Sodium Azide, BSA-Free

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2 - 8 °C

Expiry Date: 12 months

Target Details for c-MET

Target: c-MET (MET) (Met Proto-Oncogene (MET))

Alternative Name: Met

Background: The proto-oncogene MET is a member of the receptor tyrosine kinase family of proteins. The preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. [taken from NCBI Entrez Gene (Gene ID: 4233)].

Gene ID: 4233

UniProt: P08581

Pathways: RTK Signaling, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor