Recombinant HRAS antibody (AA 50-100)

Catalog No. ABIN7795745

This Rabbit Monoclonal antibody specifically detects HRAS in WB, IHC, IP, ICC and FACS. It exhibits reactivity toward Human.

Quick Overview for Recombinant HRAS antibody (AA 50-100) (ABIN7795745)

Target: HRAS (HRas proto-oncogene, GTPase (HRAS))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This HRAS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: BLR326M

Product Details anti-HRAS Antibody

Binding Specificity: AA 50-100

Purpose: Rabbit anti-N-Ras Recombinant Monoclonal Antibody [BLR326M]

Purification: Purified

Immunogen: between 50 and 100

Isotype: IgG

Application Details

Application Notes: WB,11,000,IHC,1100 to 1500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.,Flow-Cyt,Fixed in 4 % formaldehyde and permeabilized with 90 % methanol. 1 μL per 1 x 10^6 cells.,ICC,1100 to 1500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.,IP,12 μL/mg lysate

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: Phosphate Buffered Saline (PBS) with 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2 - 8 °C

Expiry Date: 12 months

Target Details for HRAS

Target: HRAS (HRas proto-oncogene, GTPase (HRAS))

Alternative Name: N-Ras

Background: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. N-Ras plays an important role in the regulation of cell proliferation and plays a role in promoting oncogenic events and mutations in N-Ras have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [taken from NCBI Entrez Gene (Gene ID: 4893)].

Gene ID: 4893

UniProt: P01111

Pathways: p53 Signaling, MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling, BCR Signaling