Recombinant p21 antibody (AA 100-150)

Catalog No. ABIN7795764

The Rabbit Monoclonal anti-p21 antibody is suitable to detect p21 in samples from Human. It has been validated for WB, IHC, IP and ICC.

Quick Overview for Recombinant p21 antibody (AA 100-150) (ABIN7795764)

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This p21 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: BLR337M

Product Details anti-p21 Antibody

Binding Specificity: AA 100-150

Purpose: Rabbit anti-p21/CIP1/CDKN1A Recombinant Monoclonal Antibody [BLR337M]

Purification: Purified

Immunogen: between 100 and 150

Isotype: IgG

Application Details

Application Notes: ICC,1100 to 1500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.,IHC,1100 to 1500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.,WB,11,000,IP,12 μL/mg lysate

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: Phosphate Buffered Saline (PBS) with 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2 - 8 °C

Expiry Date: 12 months

Target Details for p21

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Alternative Name: p21/CIP1/CDKN1A

Background: P21/CIP1/CDKN1A is a potent cyclin-dependent kinase inhibitor. It binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. Expression of p21/CIP1/CDKN1A is tightly controlled by the tumor suppressor protein p53, and it mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. p21/CIP1/CDKN1A can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. [taken from NCBI Entrez Gene (Gene ID: 1026)].

Gene ID: 1026

UniProt: P38936

Pathways: p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy