Recombinant GFP antibody (AA 1-238) (FL550)

Catalog No. ABIN7830379

This Chicken Chimeric antibody specifically detects GFP in IHC and ICC. It exhibits reactivity toward Jellyfish.

Quick Overview for Recombinant GFP antibody (AA 1-238) (FL550) (ABIN7830379)

Target: GFP (Green Fluorescent Protein (GFP))

Antibody Type: Recombinant Antibody

Reactivity: Jellyfish

Host: Chicken, Mouse

Clonality: Chimeric

Conjugate: This GFP antibody is conjugated to FL550

Application: Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Clone: N86-38

Product Details anti-GFP Antibody (FL550)

Binding Specificity: AA 1-238

Expression System: Mammalian cells

Purpose: Anti-GFP (N86/38) Recombinant Chicken Chimeric mAb FL550 Conjugate

Cross-Reactivity (Details): Cross-reacts with Cyan and Yellow fluorescent protein (CFP and YFP, respectively)Does not cross-react with Red fluorescent protein (dsRed)

Characteristics: This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N86/38 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N86/38 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media and conjugated.

Purification: Purified by affinity chromatography

Immunogen: Fusion protein amino acids 1-238 (full-length) of jellyfish green fluorescent protein (accession number P42212) produced recombinantly in E. Coli

Isotype: IgY

Application Details

Application Notes: 1:500-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 1X PBS, 0.05 % Sodium Azide pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Expiry Date: 24 months

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP

Background:

Green fluorescent protein

Our chicken Anti-GFP FL550 conjugate recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N86/38. It detects n/a GFP and is purified by affinity chromatography. It works in IHC, ICC.

GFP

Molecular Weight: 27 kDa

UniProt: P42212