CD47 antibody (FITC)

Catalog No. ABIN7836400

The Rat Monoclonal anti-CD47 antibody is suitable to detect CD47 in samples from Mouse. It has been validated for FACS and IF.

Quick Overview for CD47 antibody (FITC) (ABIN7836400)

Target: CD47

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD47 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: Miap301

Product Details anti-CD47 Antibody (FITC)

Purpose: Rat monoclonal antibody FITC labled to CD47

Specificity: Recognizes mouse CD47

Purification: The antibody was purified by affinity chromatography.

Application Details

Application Notes: Flow: 10 μL / assay

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Shipped and store at 4°C for one year. Do not freeze.

Target Details for CD47

Target: CD47

Alternative Name: CD47

Background: MER6, Leukocyte surface antigen CD47, Antigenic surface determinant protein OA3, Integrin-associated protein, IAP, Protein MER6, CD47

Gene ID: 16423