CD47 antibody (PE)

Catalog No. ABIN7836401

The PE-conjugated Mouse Monoclonal anti-CD47 antibody (Clone CC2C6D4) (ABIN7836401) specifically detects CD47 in FACS and IF. The antibody is reactive with Human samples.

Quick Overview for CD47 antibody (PE) (ABIN7836401)

Target: CD47

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD47 antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: CC2C6D4

Product Details anti-CD47 Antibody (PE)

Purpose: Mouse monoclonal antibody PE labled to CD47

Specificity: Recognizes human CD47

Purification: The antibody was purified by affinity chromatography.

Immunogen: Native purified human CD47.

Application Details

Application Notes: Flow: 10 μL / assay

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Shipped and store at 4°C for one year. Do not freeze.

Target Details for CD47

Target: CD47

Alternative Name: CD47

Background: MER6, Leukocyte surface antigen CD47, Antigenic surface determinant protein OA3, Integrin-associated protein, IAP, Protein MER6, CD antigen CD47

Gene ID: 961

UniProt: Q08722