IGKV1-27 antibody (AA 241-356)

Catalog No. ABIN7859624

This Mouse Monoclonal antibody specifically detects IGKV1-27 in IHC. It exhibits reactivity toward Human.

Quick Overview for IGKV1-27 antibody (AA 241-356) (ABIN7859624)

Target: IGKV1-27 (Immunoglobulin kappa Variable 1-27 (IGKV1-27))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IGKV1-27 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: TNFAIP3-2813

Product Details anti-IGKV1-27 Antibody

Binding Specificity: AA 241-356

Purpose: A20 / TNFAIP3 (Marker of Tamoxifen Resistance) Antibody

Specificity: A20 (also known as TNFAIP3) is a zinc finger protein and inhibits NF-kappa B activation as well as TNF-mediated apoptosis. The protein has both ubiquitin ligase and deubiquitinase activities, is involved in the cytokine-mediated immune and inflammatory responses. A20 is as a tumor suppressor and has been shown to protect MCF-7 breast carcinoma cells from TNF-induced apoptosis. HighA20expression levels were observed in more aggressive breast tumors (ER-negative, progesterone receptor-negative and high histological grade). These findings strongly suggest that A20 is a key protein involved in tamoxifen resistance.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa 241-356) of human TNFAIP3 protein (exact sequence is proprietary)

Isotype: IgG2c, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for IGKV1-27

Target: IGKV1-27 (Immunoglobulin kappa Variable 1-27 (IGKV1-27))

Alternative Name: A20

Background:

A20, Putative DNA-binding protein A20, TNF alpha-induced protein 3 (TNFAIP3), Tumor Necrosis Factor alpha-Induced Protein 3(TNFAIP3), Zinc finger protein A20

Hodgkin s Lymphoma or hepatocellular carcinoma (HCC).

Molecular Weight: ~90kDa

Gene ID: 7128, 211600

UniProt: P21580

Pathways: Activation of Innate immune Response