Recombinant CD45RA antibody

Catalog No. ABIN7863306

The Mouse Monoclonal anti-CD45RA antibody is suitable to detect CD45RA in samples from Human. It has been validated for IHC and WB.

Quick Overview for Recombinant CD45RA antibody (ABIN7863306)

Target: CD45RA

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD45RA antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Clone: RPTPRC-1131

Product Details anti-CD45RA Antibody

Purpose: Recombinant CD45RA (Leucocyte Marker) Antibody

Specificity: Recognizes a protein of 205 kDa-220 kDa, identified as CD45RA. CD45RA is isoforms of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50 % of peripheral CD4+ T-cells, 50 % of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human PTRPC protein

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD45RA

Target: CD45RA

Alternative Name: CD45RA

Background:

B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly-5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein

Daudi, IM9 or human lymphocytes. Human spleen, lymph nodes or tonsils., Raji, Ramos.

Molecular Weight: 205-220kDa

Gene ID: 5788, 654514

UniProt: P08575