MGMT antibody

Catalog No. ABIN7863130

The Mouse Monoclonal anti-MGMT antibody is suitable to detect MGMT in samples from Human. It has been validated for WB, IHC and IF.

Quick Overview for MGMT antibody (ABIN7863130)

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MGMT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: MGMT-4791

Product Details anti-MGMT Antibody

Purpose: MGMT (Marker of Suppressor of Mutation and Carcinogenesis) Antibody

Specificity: Cancer chemotherapeutic alkylating agents (e.g. BCNU,) act by inducing formation of lethal cross links at the O6 alkylguanine position in DNA. MGMT transfers alkyl adducts from the O6 position of guanine in DNA (prior to cross link formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible, hence the level of MGMT in a cell is directly proportional to the level of DNA damage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely to be drug resistant

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Purified His-tagged MGMT protein

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for MGMT

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Alternative Name: MGMT

Background:

6 O methylguanine DNA methyltransferase, Methylated DNA protein cysteine methyltransferase, O 6 methylguanine DNA alkyltransferase, O 6 methylguanine DNA methyltransferase

Jurkat or MCF-7 cells. Human tonsil or stomach (IHC).

Molecular Weight: 21.646 kDa

Gene ID: 4255, 501522

UniProt: P16455

Pathways: DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus