Recombinant MGMT antibody

Catalog No. ABIN7861875

The Rabbit Monoclonal anti-MGMT antibody is suitable to detect MGMT in samples from Human. It has been validated for WB and IHC.

Quick Overview for Recombinant MGMT antibody (ABIN7861875)

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This MGMT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: MGMT-8319R

Product Details anti-MGMT Antibody

Purpose: Recombinant MGMT (Marker of Suppressor of Mutation and Carcinogenesis) Antibody

Specificity: Cancer chemotherapeutic alkylating agents (e.g. BCNU,) act by inducing formation of lethal cross 79oΩlinks at the O6 79oΩ alkylguanine position in DNA. MGMT transfers alkyl adducts from the O6 79oΩposition of guanine in DNA (prior to cross 79oΩlink formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible, hence the level of MGMT in a cell is directly proportional to the level of DNA 79oΩdamage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely to be drug resistant

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Purified His-tagged MGMT protein

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for MGMT

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Alternative Name: MGMT

Background:

6 O methylguanine DNA methyltransferase, Methylated DNA protein cysteine methyltransferase, O 6 methylguanine DNA alkyltransferase, O 6 methylguanine DNA methyltransferase

MCF-7, LNCaP, Hep G2 and human liver, Jurkat. Human tonsil or stomach (IHC).

Molecular Weight: 21.646 kDa

Gene ID: 4255, 501522

Pathways: DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus