PCNA antibody

Catalog No. ABIN7863213

The Mouse Monoclonal anti-PCNA antibody (Clone PC10) (ABIN7863213) specifically detects PCNA in WB, IHC and FACS. The antibody is reactive with Human, Mouse, Rat, Chicken, Pig, Monkey, Saccharomyces cerevisiae, Rabbit, Zebrafish (Danio rerio), Drosophila melanogaster, Schizosaccharomyces pombe, Hamster and Guinea Pig samples.

Quick Overview for PCNA antibody (ABIN7863213)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivity: Human, Mouse, Rat, Chicken, Pig, Monkey, Saccharomyces cerevisiae, Rabbit, Zebrafish (Danio rerio), Drosophila melanogaster, Schizosaccharomyces pombe, Hamster, Guinea Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This PCNA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: PC10

Product Details anti-PCNA Antibody

Purpose: PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Antibody

Specificity: Recognizes a non-histone protein of 36 kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Rat PCNA/protein A fusion protein

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name: PCNA

Background:

Cyclin, DNA polymerase delta auxiliary protein, Mutagen-sensitive 209 protein, PCNAR, Polymerase delta accessory protein

A-431, 293T, Hep G2, MCF7, NIH/3T3, A549, Raji cells, Human Tonsil, Human Spleen or kidney

Molecular Weight: 36kDa

Gene ID: 5111, 147433, 728886

UniProt: P12004

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy