Recombinant PCNA antibody

Catalog No. ABIN7864383

This Mouse Monoclonal antibody specifically detects PCNA in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant PCNA antibody (ABIN7864383)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PCNA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: RPCNA-8859

Product Details anti-PCNA Antibody

Purpose: Recombinant PCNA (Proliferating Cell Nuclear Antigen) (G1- & S-phase Marker) Antibody

Specificity: Recognizes a non-histone protein of 36 kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human PCNA protein

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name: PCNA

Background:

Cyclin, DNA polymerase delta auxiliary protein, Mutagen-sensitive 209 protein, PCNAR, Polymerase delta accessory protein

A-431, 293T, Hep G2, NIH/3T3, Raji cells, Human Tonsil, A549, HeLa, HEK293, K-562, Raw.

Molecular Weight: 36kDa

Gene ID: 5111

UniProt: P12004

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy