Recombinant BRAF antibody

Catalog No. ABIN7865334

This Rabbit Monoclonal antibody specifically detects BRAF in IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant BRAF antibody (ABIN7865334)

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This BRAF antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: BRAF-V600E-13276R

Product Details anti-BRAF Antibody

Purpose: Recombinant BRAF (V600E Mutant Specific) (Prognostic Marker) Antibody

Specificity: The BRAF gene encodes a protein that is part of the RAS-RAF-MEK-ERK signaling pathway, which regulates cell division and proliferation. The V600E mutation in the BRAF gene leads to the production of a constitutively active BRAF protein, resulting in uncontrolled cell growth and division. Identifying the presence of this mutation is crucial for diagnosing and guiding the treatment of certain cancers. The BRAF (V600E) antibody is used in immunohistochemistry to detect the presence of a specific mutation in the BRAF gene known as V600E. This mutation is commonly associated with various cancers, including melanoma, colorectal cancer, and certain types of thyroid cancer, lung cancer, and Hairy cell leukemia. The BRAF (V600E) antibody specifically binds to the mutated BRAF protein, allowing pathologists to detect the mutation in cancer tissue. Immunohistochemistry using this antibody is often employed in the evaluation of tumor specimens to aid in the diagnosis and classification of cancers.

Purification: 200ug/ml of Ab purified from rabbit anti-serum by Protein A.

Immunogen: Recombinant fragment corresponding to human BRAF V600E mutant protein

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for BRAF

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Alternative Name: BRAF

Background:

FLJ95109, 94 kDa B raf protein, B Raf proto oncogene, serine/threonine kinase, B RAF1, B-Raf proto-oncogene serine/threonine-protein kinase (p94), BRAF 1, BRAF, BRAF1, cRmil, MGC126806, MGC138284, Murine sarcoma viral (v-raf) oncogene homolog B1, NS7, oncogene BRAF1, p94, Proto-oncogene B-Raf, Proto-oncogene c-Rmil, RAFB1, RMIL, Serine/threonine-protein kinase B-raf, v-Raf murine sarcoma viral oncogene homolog B1

A431, Jurkat or HeLa cells. Human melanoma, brain, testis or colon carcinoma.

Molecular Weight: 35kDa (predicted)

Gene ID: 673, 550061

UniProt: P15056

Pathways: MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy