Retinoblastoma 1 antibody (pSer807)

Catalog No. ABIN7862497

The Rabbit Polyclonal anti-Retinoblastoma 1 antibody (ABIN7862497) specifically detects Retinoblastoma 1 in WB, IHC, ELISA and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Retinoblastoma 1 antibody (pSer807) (ABIN7862497)

Target: Retinoblastoma 1 (RB1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Retinoblastoma 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Retinoblastoma 1 Antibody

Binding Specificity: pSer807

Purpose: Rabbit Anti-Rb (phospho Ser807) Polyclonal Antibody

Specificity: Phospho-Rb (S807) Polyclonal Antibody detects endogenous levels of Rb protein only when phosphorylated at S807.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Rb Phospho-Ser807

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Retinoblastoma 1

Target: Retinoblastoma 1 (RB1)

Alternative Name: Rb

Background: RB1, Retinoblastoma-associated protein, p105-Rb, pRb, Rb, pp110RB transcriptional corepressor 1 encoded by RB1 is a negative regulator of the cell cycle and was the first tumor suppressor gene found. RB transcriptional corepressor 1 also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in RB1 are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.

Molecular Weight: 106kD

Gene ID: 5925

UniProt: P06400

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy