MAP3K1 antibody (AA 1211-1310)

Catalog No. ABIN7865694

The Mouse Monoclonal anti-MAP3K1 antibody is suitable to detect MAP3K1 in samples from Human. It has been validated for WB, ELISA, IHC, IF, ICC and FACS.

Quick Overview for MAP3K1 antibody (AA 1211-1310) (ABIN7865694)

Target: MAP3K1 (Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAP3K1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: EBS-T-008

Product Details anti-MAP3K1 Antibody

Binding Specificity: AA 1211-1310

Purpose: anti-MAP3K1 mouse monoclonal, EBS-T-008, purified

Purification: affinity chromatography

Immunogen: partial recombinant MAP3K1 (aa1211-1310)

Isotype: IgG2a kappa

Application Details

Application Notes:

ICC/IF_dilution: assay dependent

FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml

IHC-paraffin_dilution: 1:100 - 1:200 (0,5-1,0 μg/mL, microwave treatment in 10 mM Tris with 1 mM EDTA pH 9.0 recommended)

WB_dilution: 1:100-1:200 (0.5-1 μg/mL)

IHC-frozen_dilution: 1:100 - 1:200 (0,5-1,0 μg/mL )

elisa_dilution: assay dependent

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details for MAP3K1

Target: MAP3K1 (Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1))

Alternative Name: MAP3K1

Background: MAPKs are involved in directing cellular responses to a diverse array of potentially harmful stimuli, such as mitogens, osmotic stress, heat shock, proinflammatory cytokines, but also growth factors (mammals). Possibly located exclusively in the cell nucleus, they regulate cell functions including proliferation, gene expression, differentiation, mitosis, cell survival, and apoptosis. In order to become active, they require usually multiple phosphorylation events in their activation loops, including phosphorylation by MAP2 kinases (Ste-7 kinases), which in turn are phosphorylated by the MAP3 kinase family, of which many are located at the cell membrane. Thus through this pathway, stimuli can effectively be conveyed from the cell membrane to the nucleus. Inactivation of MAPKs takes place by several phosphorylases, including dedicated phophorylases. Positive control: A431, HeLa or HL-60 cells or liver tissue.

Pathways: MAPK Signaling, Interferon-gamma Pathway, Caspase Cascade in Apoptosis, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Regulation of Actin Filament Polymerization, Toll-Like Receptors Cascades