AIM2 antibody (AA 136-354)

Catalog No. ABIN7870555

The Rabbit Polyclonal anti-AIM2 antibody (ABIN7870555) specifically detects AIM2 in WB, ELISA and FACS. The antibody is reactive with Mouse samples.

Quick Overview for AIM2 antibody (AA 136-354) (ABIN7870555)

Target: AIM2 (Absent in Melanoma 2 (AIM2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AIM2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-AIM2 Antibody

Binding Specificity: AA 136-354

Purpose: Aim2 Antibody

Purification: Antigen affinity purified

Immunogen: Recombinant mouse Aim2 protein (amino acids Q136-E354) was used as the immunogen for the Aim2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Aim2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Aim2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for AIM2

Target: AIM2 (Absent in Melanoma 2 (AIM2))

Alternative Name: Aim2

Background: Interferon-inducible protein AIM2, also known as absent in melanoma 2 or simply AIM2,is a protein that in humans is encoded by the AIM2 gene. It is mapped to 1q22. AIM2 is a member of the Ifi202/IFI16 family. It plays a putative role in tumorigenic reversion and may control cell proliferation. Interferon-gamma induces expression of AIM2. Though there has been virtually no biochemistry performed, a model based on cell-based or in vivo experiments has led to the current model of how AIM2 triggers the inflammasome. The C-terminal HIN domain binds double stranded DNA (either viral, bacterial, or even host) and acts as a cytosolic dsDNA sensor. This leads to the oligomerization of the inflammasome complex. The N-terminal pyrin domain of AIM2 interacts with the pyrin domain of another protein ASC (or Apoptosis-associated Speck-like protein containing a caspase activation and recruitment domain). ASC also contains a CARD domain (caspase activation and recruitment domain), that recruits procaspase-1 to the complex. This leads to the autoactivation of caspase-1, an enzyme that processes proinflammatory cytokines (IL-1b and IL-18).

UniProt: Q91VJ1

Pathways: Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Inflammasome