Caspase 8 antibody (AA 217-479)

Catalog No. ABIN7872250

The Rabbit Polyclonal anti-Caspase 8 antibody (ABIN7872250) specifically detects Caspase 8 in WB, ELISA and FACS. The antibody is reactive with Human samples.

Quick Overview for Caspase 8 antibody (AA 217-479) (ABIN7872250)

Target: Caspase 8 (CASP8)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-Caspase 8 Antibody

Binding Specificity: AA 217-479

Purpose: Caspase-8 Antibody / CASP8

Purification: Antigen affinity purified

Immunogen: Recombinant human protein (amino acids S217-D479) was used as the immunogen for the Caspase-8 antibody. This sequence includes both the large and small subunit domains.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Caspase-8 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Caspase-8 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Caspase 8

Target: Caspase 8 (CASP8)

Alternative Name: Caspase-8

Background: CASP8 is also known as CAP4, MACH or MCH5. This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. In addtion, this protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.

UniProt: Q14790

Pathways: Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades