PUMA antibody

Catalog No. ABIN7880054

This Rabbit Polyclonal antibody specifically detects PUMA in WB. It exhibits reactivity toward Human.

Quick Overview for PUMA antibody (ABIN7880054)

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PUMA antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PUMA Antibody

Purpose: PUMA Antibody (alpha/beta) / BBC3

Sequence: EQWAREIGAQ LRRMADDLNA QYE

Purification: Antigen affinity purified

Immunogen: Amino acids EQWAREIGAQLRRMADDLNAQYE were used as the immunogen for the PUMA antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PUMA antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PUMA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PUMA

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Alternative Name: PUMA

Background: Bcl-2-binding component 3, isoforms 1/2 (BBC3), also called p53 upregulated modulator of apoptosis (PUMA), is a pro-apoptotic member of the Bcl-2 protein family. The gene is located at 19q. The transcript is contained within 4 exons, with the presumptive initiation codon in exon 2. The predicted 193-amino acid PUMA protein shares 91 % amino acid identity with the murine sequence. Bcl-2 family members can form hetero- or homodimers, and they act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The expression of PUMA is regulated by the tumor suppressor p53, and the protein has been shown to be involved in p53-mediated apoptosis. Additionally, PUMA encodes two BH3 domain-containing proteins, PUMA-alpha and PUMA-beta, that are produced through the use of an alternative first exon and are induced in cells following p53 activation. Furthermore, PUMA couples the nuclear and cytoplasmic proapoptotic functions of p53.

UniProt: Q9BXH1

Pathways: p53 Signaling, Positive Regulation of Endopeptidase Activity