CD45RA antibody

Catalog No. ABIN7880309

This Mouse Monoclonal antibody specifically detects CD45RA in FACS, IF, WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for CD45RA antibody (ABIN7880309)

Target: CD45RA

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD45RA antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: K4B5

Product Details anti-CD45RA Antibody

Purpose: CD45RA Antibody (azide and preservative free)

Purification: Protein G affinity chromatography

Immunogen: Stimulated human leukocytes were used as the immunogen for the CD45RA antibody.

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal dilution of the CD45RA antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the CD45RA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for CD45RA

Target: CD45RA

Alternative Name: CD45RA

Background: Recognizes a protein of 205 kDa-220 kDa, identified as CD45RA. CD45RA is isoforms of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50 % of peripheral CD4+ T-cells, 50 % of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).

UniProt: P08575