CD45RA antibody

Catalog No. ABIN7880310

The Mouse Monoclonal anti-CD45RA antibody is suitable to detect CD45RA in samples from Human. It has been validated for FACS, IF, WB and IHC (p).

Quick Overview for CD45RA antibody (ABIN7880310)

Target: CD45RA

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD45RA antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: K4B5

Product Details anti-CD45RA Antibody

Purpose: CD45RA Antibody

Purification: Protein G affinity chromatography

Immunogen: Stimulated human leukocytes were used as the immunogen for the CD45RA antibody.

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal dilution of the CD45RA antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the CD45RA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for CD45RA

Target: CD45RA

Alternative Name: CD45RA

Background: Recognizes a protein of 205 kDa-220 kDa, identified as CD45RA. CD45RA is isoforms of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50 % of peripheral CD4+ T-cells, 50 % of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).

UniProt: P08575